Delivering fast track process definition
What are the expression controls?
pAVEway™ expression is based on unique protein vectors that are designed to maintain Transcription control via DNA looping. Modulation of expression is based on level of inducer added.
The technology allows for the manipulation of induction conditions to optimize accumulation allowing for maximization of protein expression as either inclusion bodies, cytoplasmic soluble or periplasmic secretion.
What Host cells are used?
pAVEway™ host cells include a panel of E.coli hosts chosen for High levels of expression of recombinant proteins and robust fermentation performance at high cell densities.
What is the pAVEway Process Fermentation Platform?
pAVEway™ process fermentation platform is based on Batch medium with a pre-developed feed strategy that delivers High ‘protein productive’ biomass levels. This platform has been proven to be scalable in the manufacturing setting.
As a well established platform Induction protocols are pre-developed, no matter how the protein is expressed:
- Inclusion body formation
- Soluble intracellular accumulation
- Secretion – periplasmic/growth medium
What are the benefits of pAVEway?
Fast track process creation and development: Reduced time to clinic, reduced time to market.
Antibiotic-free production: Antibiotic-free vectors suitable for large scale antibiotic-free cGMP manufacturing in a tightening regulatory environment without sacrificing yield and versatility.
High yield protein production through class leading fermentation productivity, with potential for reduced cost of goods.
Produces a wide range of biotherapeutic proteins, through a variety of accumulation routes; intracellular soluble, intracellular insoluble, and periplasmic secretion. The optimal route can be tailored for each individual protein.
Proven scaleability with over 100 different therapeutic proteins produced at very high titers over the past decade