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Apollo™ X: A Perspective from our Scientist

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Apollo™ X is designed to be a ‘manufacturing-ready’ cell line with a robust support package to aid IND filing. FUJIFILM Diosynth Biotechnologies’ scientists have researched the key components in the generation of a recombinant cell line and, as a result, the next generation of our Apollo™ X mammalian expression system will produce a high quality recombinant cell line to take your biopharmaceutical from pre-clinical through to commercial production efficiently and rapidly.

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WHAT ARE THE MAIN COMPONENTS OF OUR APOLLO X MAMMALIAN CELL LINE?

VECTOR

  • DHFR based selection system
  • Proprietary leader sequence developed for efficient secretion
  • High productivities achieved without the need for amplification
  • Codon optimization utilized
  • Double gene vector

Host Cell Line

  • It is a DG44 derived host cell line which was adapted to chemically-defined medium and suspension culture.
  • It was selected for superior growth characteristics and expression capability.
  • Fully characterized cGMP cell bank.

Cell Line Development Process

  • It is Animal component free media and reagents used for the entire cell line development process.
  • Product concentrations in excess of 10 g/L are typically seen using platform bioreactor conditions.

Media & feed Optimization

  • Chemically-defined
  • Selected for superior characteristics including growth, titer, and product quality
  • Concept of continuous improvement with formulation knowledge

Connect with us to see how Apollo X can advance your project, contact FUJIFILM Diosynth Biotechnologies by completing this form: